Technical Team

John SIMONET obtained his research Master’s degree in Cellular and Molecular Signaling from the University of Bourgogne Franche-Comté in 2019. During this period, he studied the impact of an HSP90 inhibitor on the LCK protein and its related signaling pathways in a T-ALL xenograft model, within Dr. GARRIDO’s team.

In 2021, he joined Dr. CALLANAN’s team, where he worked first as a technician and then as an assistant engineer, contributing to the study of the PRMT2 protein and its impact on inflammation in an AML model derived from the human HL-60 cell line. He gained expertise in molecular biology techniques such as RT-qPCR and Western blotting (including immunoprecipitation to verify protein-protein interactions), as well as Dot blotting. He also deepened his skills in cell culture, where he was responsible for monitoring the transfection of HL-60 cells to create PRMT2 knock-out or overexpression models.

In 2023, he joined the IMATHERA team under Dr. COLLIN, initially as a technician and currently as a research engineer. He has been trained in radiolabeling techniques for service provision, as well as flow cytometry methods to study, among other things, the impact of SPIONs on the polarization of murine and human macrophages. He is also involved in the technical support team to ensure the proper functioning of the laboratory (monitoring compliance with radioactive product handling in coordination with the platform’s radiation safety officers, and overseeing the operation of the BSL-2 cell culture lab).